Device
AMR_X

Part:BBa_K5099027:Design

Designed by: Isabelle Guo   Group: iGEM24_McGill   (2024-09-13)


CasX_AMR_Device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5127
    Illegal PstI site found at 4609
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5127
    Illegal PstI site found at 4609
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5127
    Illegal BglII site found at 760
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5127
    Illegal PstI site found at 4609
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5127
    Illegal PstI site found at 4609
    Illegal NgoMIV site found at 1952
    Illegal NgoMIV site found at 2225
    Illegal NgoMIV site found at 2384
    Illegal NgoMIV site found at 2837
    Illegal NgoMIV site found at 2894
    Illegal NgoMIV site found at 3767
    Illegal NgoMIV site found at 4789
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 185
    Illegal BsaI site found at 458
    Illegal SapI.rc site found at 3762


Design Notes

Integration of reporter gate with restoration gate and spacer mechanism.


Source

Engineered part from CasX system.

References